Figure 2.
Trafficking of hSK2 channels after coexpression with CaMWT or mutant CaMs.(A) HEK 293 cells expressing SK2 fusion protein with HA tag coexpressed with CaMWT or mutant CaMs (N54I, F90L, D96V, N98S, and D130G) using NP and permeabilized (P) conditions. (B) Control represents incubation with secondary antibody alone. Scale bars are 10 µm. (C) Summary data showing fluorescence intensity under NP/P conditions (555/633 fluorescent intensity). Data shown represent mean ± SEM. Statistical analyses were performed using one-way ANOVA combined with Tukey’s test. n = 15–20 cells; P= NS. DAPI, 4′,6-diamidino-2-phenylindole.

Trafficking of hSK2 channels after coexpression with CaMWT or mutant CaMs. (A) HEK 293 cells expressing SK2 fusion protein with HA tag coexpressed with CaMWT or mutant CaMs (N54I, F90L, D96V, N98S, and D130G) using NP and permeabilized (P) conditions. (B) Control represents incubation with secondary antibody alone. Scale bars are 10 µm. (C) Summary data showing fluorescence intensity under NP/P conditions (555/633 fluorescent intensity). Data shown represent mean ± SEM. Statistical analyses were performed using one-way ANOVA combined with Tukey’s test. n = 15–20 cells; P= NS. DAPI, 4′,6-diamidino-2-phenylindole.

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