Feedback model for TRPV4 cluster formation. (A) In silico images of TRPV4 clusters at 0, 12, 24, 36, and 48 h after the initiation of expression. (B) Solutions to the feedback model equations $dPRdt$⁠, $dPgdt$⁠, and $dPndt$⁠. (C and D) The black trace depicts the time course of mean cluster area (C) and density of simulated TRPV4 clusters (D). Colored dots represent the superimposed experimental data from Fig. 4. (E) Predicted mean TRPV4 channel (black) and cluster dwell times (red) based on the feedback model.