Mechanical properties of the AR chains extracted from equilibrium MD simulations. (A) Structure of ANK1 highlighting the six ARs and the second (cyan) and fifth (red) repeats used to calculate the COM-to-COM distance from AR₂ to AR₅ (d_2,5). (B) The time evolution of the distance d_2,5 from A for ANK1 (blue), ANK2 (red), and ANK3 (green). (C) The stiffness spring constant and 95% CI for k_s for ANK1–ANK3 computed from the data in B using Eq. 2. (D) Structure of ANK1 highlighting the six ARs and the second (cyan) and fifth (red) repeats used to calculate the angular displacement of AR₂ with respect to AR₅ (θ_2,5). The dots represent the COM and C_α atoms used to define AR orientations. (E) The time evolution of the angle θ_2,5 from D for ANK1 (blue), ANK2 (red), and ANK3 (green). (F) The torsional spring constant and 95% CI for k_θ for ANK1–ANK3 computed from the data in E using Eq. 3. Error bars in C and F are the 95% CI obtained using a Monte Carlo bootstrap technique.