Specific amino and carboxyl-terminal cysteine residues form cross-links with ATP pretreatment. (A) Histogram showing the average percentage dimer present for R25C to G30C tested with all three cross-linkers in the presence of 300 µM ATP. This shows that only R25C and G30C show 40–60% dimerization. (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, n = 3–5). (B) Histogram showing the average percentage dimer present for H355C to R360C tested with all three cross-linkers in the presence of 300 µM ATP. This shows that only P358C and R360C show 40–60% dimerization. (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; n = 3–5).