Comparison of HBC gate distances of PIP₂-bound and PIP₂-depleted control simulations. Combined F192 distance data of control simulations with (10 × 0.2 µs, orange color) or without (10 × 0.2 µs, black color) PIP₂ bound. Table S1 gives an overview of all control simulations. (A) Distances between opposing Cα atoms of the HBC-gate–forming residue F192. The distance observed in the crystal structure (15.3 Å) is indicated by a red cross. (B) Minimum distances between opposing F192 residues. In both panels, only the narrower subunit pair was considered at each time step of all control runs.