In silico analysis suggests that TRPV4 channel activity depends on its proximity to AKAP150. (A) Simulated images of randomly distributed TRPV4 (green) and AKAP150 (red) clusters in male and female pial (left), parenchymal (center), and mesenteric (right) myocytes. (B) Bar plot of the in vitro (measured using GSD super-resolution nanoscopy) and in silico distances between AKAP150 and TRPV4 clusters. (C) Experimentally determined (i.e., nP_o) and in silico calculated TRPV4 channel activities. (D) Plot of in vitro (nP_o; black squares and line) and in silico (red circle and line) TRPV4 channel versus the mean AKAP150 to TRPV4 distance. Solid lines show fits to the data using a single-exponential function.