Differential TRPV4 channel and AKAP150 distribution in male and female mesenteric, pial, and parenchymal myocytes. (A–C) Super-resolution images of TRPV4 (green) and AKAP150 (red) in pial (A), parenchymal (B), and mesenteric (C) myocytes. Smaller images to the right of each image show expanded views of the regions of interest (ROIs) marked by yellow squares as well as the binary version of the same ROIs (mask). Binary images were used to measure the cluster area and distance to nearest neighbor. (D) Bar plot of the mean distance ± SEM between TRPV4 and AKAP150 clusters in male (n = 750 ROIs from five cells per vascular bed) and female (n = 750 ROIs from five cells per vascular bed). ***, P < 0.001.