Sex- and vessel-specific variations in GSK-induced TRPV4 sparklet activity in smooth muscle myocytes. (A and B) Amplitude histograms of amalgamated TRPV4 sparklets in male (A) and female (B) myocytes (i.e., pial, parenchymal, and mesenteric myocytes) before (white bars) and after 100 nM GSK (gray bars). Black (control) and red (GSK) lines show the best fits of the male and female data using a multi-Gaussian function with a quantal unit of Ca²⁺ influx of 50 nM. (C and D) Bar plots of mean TRPV4 sparklet activity ± SEM (C) and density ± SEM (D) in male (n = 7–8 cells per condition, 12 animals) and female (n = 8–9 cells per condition, 16 animals) pial, parenchymal, and mesenteric myocytes. *, P < 0.05; **, P < 0.01; ***, P < 0.001.