I_TRPV4 is larger in male than in female arterial and arteriolar myocytes. (A–C) Representative I_TRPV4 obtained in myocytes from pial arteries (A), parenchymal arterioles (B), and mesenteric arteries (C) using the perforated patch/whole-cell configuration of the patch-clamp technique. I_TRPV4 was defined as the HC067047-sensitive current (1 µM; control). I_TRPV4 was obtained by subtracting current traces evoked by voltage ramps in the presence of HC067047 from traces in control conditions or in the presence of 100 nM AngII. (D) Bar plot showing mean ± SEM I_TRPV4 amplitudes at 80 mV in the presence and absence of 100 nM AngII in male (n = 5–6 cells per condition, five animals) and female (n = 5 cells per condition, six animals) pial, parenchymal, and mesenteric myocytes. *, P < 0.05; **, P < 0.01.