Figure 3.
Figure 3. Coupling summary and modal activity. (A) Interaction energy versus separation. αC distance is that between α-carbons of the mutations using GluCl as the template structure. Circles, MCA coupling free energy (open, αM2M3–TBS); squares, TMD–TBS interaction energies estimated from gating constants (see Materials and methods). Lines mark ±0.6 kcal/mol. (inset) Histograms of coupling energies for separations >15 Å (mean ± SD [n]): αM2M3–TBS, −0.68 ± 1.02 (47); all others, 0.09 ± 0.56 (87). (B) αP265A induces kinetic modes. Clusters reflect C↔O gating (O is down) and long silent periods are D(esensitized). WT clusters are homogeneous (PO = 0.88 ± 0.02; mean ± SD), αP265A are modal (0.02 ± 0.01, 0.51 ± 0.07, and 0.94 ± 0.03), and αP265A+δW57A are homogeneous (0.34 ± 0.03). δW57A eliminates modes, but αY190F and αY198A do not.

Coupling summary and modal activity. (A) Interaction energy versus separation. αC distance is that between α-carbons of the mutations using GluCl as the template structure. Circles, MCA coupling free energy (open, αM2M3–TBS); squares, TMD–TBS interaction energies estimated from gating constants (see Materials and methods). Lines mark ±0.6 kcal/mol. (inset) Histograms of coupling energies for separations >15 Å (mean ± SD [n]): αM2M3–TBS, −0.68 ± 1.02 (47); all others, 0.09 ± 0.56 (87). (B) αP265A induces kinetic modes. Clusters reflect C↔O gating (O is down) and long silent periods are D(esensitized). WT clusters are homogeneous (PO = 0.88 ± 0.02; mean ± SD), αP265A are modal (0.02 ± 0.01, 0.51 ± 0.07, and 0.94 ± 0.03), and αP265A+δW57A are homogeneous (0.34 ± 0.03). δW57A eliminates modes, but αY190F and αY198A do not.

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