Figure 3.
![Figure 3. Cytoplasmic Ca2+ signals in RyR2-WT, -R2473S, and -K4750Q expressing HEK293 cells. (A) Representative fluo-4 signals of WT and mutant cells at various [Ca2+]o (top and bottom left). (bottom right) Fraction of cells showing Ca2+ oscillations. (B) Comparison of oscillations at 2 mM [Ca2+]o among WT and mutant cells. (top) Representative fluorescence signals. (bottom left) Oscillation amplitude (F/Fmax) at peak and rest. ***, P < 0.001 versus WT peak. ###, P < 0.001 versus WT rest. (bottom right) Oscillation frequency (n/min). n = 182, 181, and 146 for WT, R2473S, and K4750Q, respectively. ***, P < 0.001 versus WT. Values shown are mean ± SEM.](https://cdn.rupress.org/rup/content_public/journal/jgp/149/2/10.1085_jgp.201611624/6/jgp_201611624_fig3.jpeg?Expires=1772576480&Signature=Q9-t4aFlJDp5OQztbICYCTA5DuVPWK8erOO63F68tnx5jn6TLX9Hj6ZSDIshuMx2mvsVIhRcM2071AK4xckxu~GPK7yA4U8rjXRV3quXIC8DvXh4OSSVLL5DMUGTN2VeecLZ0RLXCI3hGr53-rilvV6GmX3QFSigr4kNDNzWfjcFnlKFiiVGboRPH60Y3D5uKBa8DqEC4qfACzsaTqTgPTKQENXRBdZvQ67awzitbobNJplMGdAGDD7tyVHFbeIta39t5z2YzYugzCFqVh70LoSKqtytwWuCAUr2EaFqJ-HZAwMBxlDBuPP9uYC5TckC9gxkkgh-jcINA~oi3yXujA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cytoplasmic Ca2+ signals in RyR2-WT, -R2473S, and -K4750Q expressing HEK293 cells. (A) Representative fluo-4 signals of WT and mutant cells at various [Ca2+]o (top and bottom left). (bottom right) Fraction of cells showing Ca2+ oscillations. (B) Comparison of oscillations at 2 mM [Ca2+]o among WT and mutant cells. (top) Representative fluorescence signals. (bottom left) Oscillation amplitude (F/Fmax) at peak and rest. ***, P < 0.001 versus WT peak. ###, P < 0.001 versus WT rest. (bottom right) Oscillation frequency (n/min). n = 182, 181, and 146 for WT, R2473S, and K4750Q, respectively. ***, P < 0.001 versus WT. Values shown are mean ± SEM.
