Figure 3.
Figure 3. Cell unroofing is useful for a variety of adherent cell types. (A) Cartoon of unroofing approach in which cells are stained with R18 (red) after sonication. (B) Unroofed and stained ND7/23, PC-12, F-11, and HEK293T/17 cells imaged with epifluorescence microscopy. Scale bar applies to all images and is 50 µm.

Cell unroofing is useful for a variety of adherent cell types. (A) Cartoon of unroofing approach in which cells are stained with R18 (red) after sonication. (B) Unroofed and stained ND7/23, PC-12, F-11, and HEK293T/17 cells imaged with epifluorescence microscopy. Scale bar applies to all images and is 50 µm.

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