Figure 2.
Figure 2. Unroofing adhered cells produces plasma membrane sheets. (A) Cartoon of the unroofing approach. A sonicator probe is shown within 2 mm of R18-labeled, adhered cultured cells. One pulse at low power disrupts the cellular contents, leaving the ventral surface of the cell on the glass. (B and C) Example of unroofing in a field of R18-stained HEK293T/17 cells. Cells before sonication are shown in B, and the same field after unroofing is shown in C. Scale bar applies to all images and is 20 µm.

Unroofing adhered cells produces plasma membrane sheets. (A) Cartoon of the unroofing approach. A sonicator probe is shown within 2 mm of R18-labeled, adhered cultured cells. One pulse at low power disrupts the cellular contents, leaving the ventral surface of the cell on the glass. (B and C) Example of unroofing in a field of R18-stained HEK293T/17 cells. Cells before sonication are shown in B, and the same field after unroofing is shown in C. Scale bar applies to all images and is 20 µm.

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