Figure 4.
Figure 4. Ion conduction and Ca2+ block of NaK2CNG-Dm. (A; left) Sample traces of single-channel recordings of NaK2CNG-Dm and NaK2CNG-E at ±100 mV. (Right) Histogram of single-channel current at −100 mV. (B) Ion selectivity and conductance of NaK2CNG-Dm and NaK2CNG-E are compared in I-V plot. Error bars are the mean ± SEM of five independent measurements. (C) Sample traces of single-channel recordings at −100 mV at different external (bath) Ca2+ concentrations. (D) Fraction of unblocked current (I/Imax) at −100 mV as a function of Ca2+ concentration. Data are fitted into a Hill equation, with Ki of 4 ± 0.6 mM and a Hill coefficient n of 0.52 for NaK2CNG-Dm and Ki of 59 ± 10 µM and n of 0.98 for NaK2CNG-E. Error bars are mean ± SEM from five measurements.

Ion conduction and Ca2+ block of NaK2CNG-Dm. (A; left) Sample traces of single-channel recordings of NaK2CNG-Dm and NaK2CNG-E at ±100 mV. (Right) Histogram of single-channel current at −100 mV. (B) Ion selectivity and conductance of NaK2CNG-Dm and NaK2CNG-E are compared in I-V plot. Error bars are the mean ± SEM of five independent measurements. (C) Sample traces of single-channel recordings at −100 mV at different external (bath) Ca2+ concentrations. (D) Fraction of unblocked current (I/Imax) at −100 mV as a function of Ca2+ concentration. Data are fitted into a Hill equation, with Ki of 4 ± 0.6 mM and a Hill coefficient n of 0.52 for NaK2CNG-Dm and Ki of 59 ± 10 µM and n of 0.98 for NaK2CNG-E. Error bars are mean ± SEM from five measurements.

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