Serial reconstruction of the murine cpPME during neuroinflammation. (A and B) Schematics of the mouse head showing CP, ONs, olfactory bulb (OB), nasal epithelium (NE), and lymphatic vessels (LV), with cpBM positioned near olfactory foramina. (C) Coronal sections from EAE mice (day 18) reveal Lyve-1⁺ lymphatics (white), podoplanin/PDPN meninges + lymphatics (red), and DAPI⁺ nuclei (blue) across six rostral-to-caudal planes (1–6) of the OB–CP interface. CP = white dotted line. * = blood vessels. Scale bars = 750 µm (top row), 100 µm (bottom row). (D) CSF1R-eGFP reporter mice show myeloid cells (green) inside cpBM, clustering around ON bundles, and lymphatic zones during EAE. Scale bars = 100 µm. (E) Low-magnification view of the OB–CP axis reveals CP LYVE-1⁺ lymphatics adjacent to ON bundles. Scale bars = 500 µm (left panel), 200 µm (right panel). (F) High-resolution images of cpPME depicting ONs surrounded by LYVE-1⁺ lymphatics and by CSF1R-eGFP⁺ immune cells. Yellow dotted lines = outline of circular cross-sectioned ONs. Scale bars = 100 µm. Figure adapted from Laaker et al. (2025) (Fig. S3, A–C; and Fig S4, A and B).