Analysis of Gox ubiquitination. (A) Gox4KR mutant and GoxWT were both bound to Ref(2)P and present as high molecular weight ubiquitinated forms (panel IP: Ref(2)P-Myc, blot: Flag). (B) Property of Gox11KR mutant. Ref(2)P associated with Gox11KR and increased its amount (compare Ref(2)P bands with and without Gox–Flag, panel IP: Gox–Flag, blot: Flag). But caused no change in ubiquitination (panel IP: Gox–Flag, blot: Ubi). The Gox11KR phenotype was identical to that of Gox8KR. The bands of native Gox and Ref(2)P are indicated by asterisks (*, ***). Source data are available for this figure: SourceData FS4.