Gox/Osi23-interacting proteins involved in nanopore formation. (A) Mass spectrometric identification of Gox-associated proteins in S2 cells expressing Gox–Flag. An asterisk (*) indicates proteins identified from cells co-expressing Gox–Flag and Myc-Ref(2)P. The complete list of identified proteins is found in Supplementary Table S2. (B) ER localization of Gox-interacting proteins Spg, TER-94, and CG13887. Image of TER-94 was processed by image deconvolution. (C) RNAi phenotypes of Gox-interacting proteins/genes. Left: SEM image of olf bristles showing strong loss of nanopore phenotype. Right: percentage of olf bristles classified as normal (Light gray), partial (gray), and strong (black) class of nanopore loss phenotype. n indicates the number of scored bristles. No formal statistical test was applied because the data represent categorical frequency counts. Scale bars: 10 µm (B) and 1 µm (C).