Plasma membrane landscape of the olf hair cell. (A) Plasma membrane structures of the olf and spinule obtained by FIB-SEM. Outer view (left) and inner view (right). (B) Longitudinal TEM views of the plasma membrane and envelope at 44, 50, and 52 h APF. (C) Envelope lengths were measured between adjacent low points in TEM images. Data points represent individual envelope fragments from n = 1–3 biological replicates per time point. A total of 95–183 fragments (5–9 TEM sections per condition) were analyzed. Data are shown as mean ± SD, and statistical significance between y w and gox KO was determined by the Mann–Whitney U test (two-sided). *: P < 0.05. n.s., not significant. (D) Quantification of envelope curvature (µm-1). Median point curvature per fragment was plotted in violin plots (n = 1–3 biological replicates). Statistical comparison used the Mann–Whitney U test (two-sided). (E) ER and Gox distributions in atl RNAi. (F) FIB-SEM plasma membrane views of atl RNAi hair cell #1. Bar: 1 μm (A, E, and F), 200 nm (B and E enlarged), and 200 nm (A and F inner side, approx.).