Knockdown of the ATG genes causes ER overexpansion, Gox mislocalization, and nanopore loss. (A) ER distribution in gox mutants, and ATG1, Atg2, Atg5 and ATG18a RNAi. Graphs in the right show averaged line scan intensities of ER or Gox along the cross section of each hair (5–7 hairs for each genotype). Line intensity profiles were obtained using the PlotProfile function in Fiji and averaged after normalization with custom Python scripts. In the gox KO panel (top), gox mutant scan is shown in magenta, and control scan in gray. For other graphs, only mutant scan data are shown. (B) HA–Gox distribution in ATG1, ATG2, ATG5, and ATG18a RNAi showing the reduction of subcortical Gox. (C) olf hairs of ATG RNAi experiments. All showed reduced nanopores compared with the control. Bar: 1 µm.