Figure 1.
ER-localized Gox/Osi23 controls ER-phagy. (A–C) TEM of the olf hair cell at 44 h APF. (A) An enlarged view of the curved cuticular envelop and the underlying plasma membrane. The lowest point of the curved envelope is often associated with PMI. (B) Localization of APEX2-Gox protein in tubular ER. Left: low-magnification view (composite of two images). Right: enlarged view of the perinuclear (orange) and the shaft region (blue). Dotted lines outline plasma membrane of the olf hair cell. (C) Three types of Gox-containing organelles. I, ER. II, electron-dense vesicle (arrowhead in B). IIIa, IIIb, electron-lucent structures. IIIa is a multi-membrane structure. (D) Proximity of Gox+ structures to the high (orange) or low (blue) point of the envelope. (E) Distance from the membrane of the three types of Gox vesicles. Median distance is shown above each plot. (F) Presumed order of Gox-vesicle conversion. (G) Localization of Gox and ER marker Cnx. (H and I) Distribution of ER (magenta) in control (y w) and gox mutant. Segmented view of FIB-SEM stacks. (J and K) ER distribution in control and ATG8a RNAi. (L) Reduction of subcortical Gox localization in ATG8a RNAi. Graphs below show averaged line scan intensities of ER or Gox along the cross section of each hair (control: gray; mutant: magenta, 5–7 hairs for each genotype). (M) Adult olf bristle of neur > ATG8a RNAi lacking nanopores (top) and control (UAS-ATG8a RNAi only, bottom). For panel E, a total of 196 Gox-positive vesicles (type I = 72, type II = 94, and type III = 30) were classified by morphology and distance from the plasma membrane. The high- and low-PM curvature points were manually identified. No formal statistical test was applied because the data represent categorical frequency counts. For panels J–L, line intensity profiles of ER and HA–Gox signals were obtained from 5–7 individual hairs per genotype, normalized, and averaged using custom Python scripts. No formal statistical test was applied; error shading represents mean ± SD. Bar: 100 nm (A), 2 µm (B), 500 nm (enlarged in B), 200 nm (C), and 1 µm (G–M).

ER-localized Gox/Osi23 controls ER-phagy. (A–C) TEM of the olf hair cell at 44 h APF. (A) An enlarged view of the curved cuticular envelop and the underlying plasma membrane. The lowest point of the curved envelope is often associated with PMI. (B) Localization of APEX2-Gox protein in tubular ER. Left: low-magnification view (composite of two images). Right: enlarged view of the perinuclear (orange) and the shaft region (blue). Dotted lines outline plasma membrane of the olf hair cell. (C) Three types of Gox-containing organelles. I, ER. II, electron-dense vesicle (arrowhead in B). IIIa, IIIb, electron-lucent structures. IIIa is a multi-membrane structure. (D) Proximity of Gox+ structures to the high (orange) or low (blue) point of the envelope. (E) Distance from the membrane of the three types of Gox vesicles. Median distance is shown above each plot. (F) Presumed order of Gox-vesicle conversion. (G) Localization of Gox and ER marker Cnx. (H and I) Distribution of ER (magenta) in control (y w) and gox mutant. Segmented view of FIB-SEM stacks. (J and K) ER distribution in control and ATG8a RNAi. (L) Reduction of subcortical Gox localization in ATG8a RNAi. Graphs below show averaged line scan intensities of ER or Gox along the cross section of each hair (control: gray; mutant: magenta, 5–7 hairs for each genotype). (M) Adult olf bristle of neur > ATG8a RNAi lacking nanopores (top) and control (UAS-ATG8a RNAi only, bottom). For panel E, a total of 196 Gox-positive vesicles (type I = 72, type II = 94, and type III = 30) were classified by morphology and distance from the plasma membrane. The high- and low-PM curvature points were manually identified. No formal statistical test was applied because the data represent categorical frequency counts. For panels J–L, line intensity profiles of ER and HA–Gox signals were obtained from 5–7 individual hairs per genotype, normalized, and averaged using custom Python scripts. No formal statistical test was applied; error shading represents mean ± SD. Bar: 100 nm (A), 2 µm (B), 500 nm (enlarged in B), 200 nm (C), and 1 µm (G–M).

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