Figure S4. Analysis of the transgenic... | Rockefeller University Press

Figure S4.

Analysis of the transgenic expression of HA-Abi and its variants. (A) Confocal images of primary hemocytes from HmlΔ-GAL4,abi5/UAS-HA-abi,Df (abi5/Df, HmlΔ > abi), UAS-HA-abiΔ30–65/+; HmlΔ-GAL4,abi5/+,Df (abi5/Df, HmlΔ > abiΔ30–65), HmlΔ-GAL4,abi5/UAS-HA-abiW452K,Df (abi5/Df, HmlΔ > abiW452K), HmlΔ-GAL4,abi5/UAS-HA-abi4YF,Df (abi5/Df, HmlΔ > abi4YF), and HmlΔ-GAL4,abi5/UAS-HA-abi4YE,Df (abi5/Df, HmlΔ > abi4YE) late-third instar larvae, stained with anti-HA (pseudocolored magenta) and anti-P1(green) antibodies. (B) Quantification of the ratio of mean anti-HA to anti-P1 fluorescence intensities. Values are the percentages of the abi5/Df, HmlΔ > abi control. n = 9 cells. Data represent the mean ± SEM. Statistical analyses were performed using a one-way ANOVA with the Tukey–Kramer post hoc test (ns, not significant). Scale bar: 10 μm.

Analysis of the transgenic expression of HA-Abi and its variants. (A) Confocal images of primary hemocytes from HmlΔ-GAL4,abi⁵/UAS-HA-abi,Df (abi⁵/Df, HmlΔ > abi), UAS-HA-abi^Δ30–65/+; HmlΔ-GAL4,abi⁵/+,Df (abi⁵/Df, HmlΔ > abi^Δ30–65), HmlΔ-GAL4,abi⁵/UAS-HA-abi^W452K,Df (abi⁵/Df, HmlΔ > abi^W452K), HmlΔ-GAL4,abi⁵/UAS-HA-abi^4YF,Df (abi⁵/Df, HmlΔ > abi^4YF), and HmlΔ-GAL4,abi⁵/UAS-HA-abi^4YE,Df (abi⁵/Df, HmlΔ > abi^4YE) late-third instar larvae, stained with anti-HA (pseudocolored magenta) and anti-P1(green) antibodies. (B) Quantification of the ratio of mean anti-HA to anti-P1 fluorescence intensities. Values are the percentages of the abi⁵/Df, HmlΔ > abi control. n = 9 cells. Data represent the mean ± SEM. Statistical analyses were performed using a one-way ANOVA with the Tukey–Kramer post hoc test (ns, not significant). Scale bar: 10 μm.

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