Analysis of cell death in abi mutant hemocytes. (A) Confocal images of primary LG lobes from WT and abi⁵/Df late-third instar larvae carrying HmlΔ-GAL4 together with UAS-EGFP (green), stained with an anti-Lz antibody (blue) and PI (pseudocolored magenta). Arrowheads indicate Lz⁺PI⁺ crystal cells. (B) Percentage of Lz⁺PI⁺ cells among total Lz⁺ crystal cells in the LG and peripheral hematopoietic compartment (PC). Lz⁺PI⁺ cells were classified as Hml⁺ (green) or Hml⁻ (blue). n = 9 lobes/larvae. Data represent the mean ± SEM. Statistical analyses were performed using Student’s t test. Comparisons are with WT (***P < 0.001; ns, not significant). Scale bar: 20 μm.