Loss of Abi reduces the number of Hml/eater lineage–traced crystal cells in the peripheral hemocyte compartment and the LG. (A) Live mount images of WT and abi⁵/Df late-third instar larvae carrying HmlΔ-DsRed. The overall distribution of DsRed⁺ hemocytes is normal in abi⁵/Df larvae compared with WT larvae. The red box indicates the dorsal hemocyte cluster in abdominal segment A7. (B and C) Confocal images of the dorsal hemocyte cluster in abdominal segment A7 of WT and abi⁵/Df late-third instar larvae carrying HmlΔ-GAL4 (B) or eater-GAL4 (C) together with UAS-G-TRACE (GFP in green and RFP in pseudocolored magenta), stained with an anti-Lz antibody (blue). (D) Numbers of Hml and eater lineage–traced cells in larvae at 60, 78, 92, and 112 h AEL at 25°C, corresponding to the second instar (L2), and early (e)-, mid (m)-, and late (l)-third instar (L3) stages, respectively. G-TRACE–labeled (G-TRACE⁺) cells were quantified as the total number of GFP⁺ only (green), RFP⁺ plus GFP⁺ (yellow), and RFP⁺-only (red) cells. (E) Numbers of Lz⁺ crystal cells at the indicated larval stages. Lz⁺ crystal cells were counted and classified as G-TRACE⁺ and G-TRACE^-. (F) Confocal images of primary LG lobes from WT and abi⁵/Df late-third instar larvae carrying HmlΔ-GAL4 together with UAS-G-TRACE, stained with an anti-Lz antibody. The dashed line outlines the LG lobe. (G) Numbers of Hml lineage–traced cells in primary LG lobes at the indicated larval stages. (H) Numbers of Lz⁺ crystal cells in primary LG lobes at the indicated larval stages. Data represent the mean ± SEM. n = 12 larvae/LG lobes. Statistical analyses were performed using Student’s t test. Comparisons are with WT (*P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant). Scale bars: 500 μm (A); 50 μm (B and C); 20 μm (F).