Figure 1.
Abi is required for maintenance of crystal cell and lamellocyte homeostasis during larval development. (A) Single confocal slices of peripheral hemocytes from WT and abi5/Df(3R)su(Hw)7 (abi5/Df) late-third instar larvae stained with an anti-P1 antibody (green) and DAPI (blue). (B) Percentage of P1+ plasmatocytes among all peripheral hemocytes (total DAPI count). (C) Confocal images of peripheral hemocytes from WT, abi5/Df, and HmlΔ-GAL4,abi5/UAS-HA-abi,Df (abi5/Df, HmlΔ > abi) late-third instar larvae stained with an anti-Lz antibody (green) and DAPI (blue). Arrowheads indicate Lz+ crystal cells. (D) Percentage of Lz+ crystal cells among all peripheral hemocytes (total DAPI count). abi5/Df; eater > abi and abi5/Df; lz > abi represent eater-GAL4,abi5/UAS-HA-abi,Df and lz-GAL4/+; UAS-HA-abi,Df/abi5, respectively. (E) Bright-field images of heated (70°C, 10 min) WT, abi5/Df, and abi5/Df, HmlΔ > abi late-third instar larvae. Dorsal views of the two most posterior segments (A7 and A8). (F) Numbers of heat-blackened crystal cells in the A7 and A8 segments of the indicated genotypes. (G) Confocal images of peripheral hemocytes from WT, abi5/Df, and abi5/Df, HmlΔ > abi late-third instar larvae stained with an anti-L1 antibody (green) and DAPI (blue). Arrowheads indicate L1+ lamellocytes. (H) Percentage of L1+ lamellocytes among all peripheral hemocytes (total DAPI count). abi5/Df, MSNF9 > abi represents MSNF9-GAL4/+; UAS-HA-abi,Df/abi5. (I) Confocal images of primary LG lobes from WT, abi5/Df, and abi5/Df, HmlΔ > abi late-third instar larvae stained with an anti-Lz antibody (green). Optical sections through the middle and two adjacent planes were projected. The dashed lines outline the MZ and CZ of the LG. (J) Numbers of Lz+ crystal cells in the primary LG lobes for the indicated genotypes. Data represent the mean ± SEM. n = 12 larvae/LG lobes. Statistical analyses were performed using Student’s t test (B) or a one-way ANOVA with the Tukey–Kramer post hoc test (D, F, H, and J). Comparisons are with WT (***P < 0.001; ns, not significant). Scale bars: 20 μm (A, C, G, and I); 200 μm (E). MZ, medullary zone; CZ, cortical zone.

Abi is required for maintenance of crystal cell and lamellocyte homeostasis during larval development. (A) Single confocal slices of peripheral hemocytes from WT and abi5/Df(3R)su(Hw)7 (abi5/Df) late-third instar larvae stained with an anti-P1 antibody (green) and DAPI (blue). (B) Percentage of P1+ plasmatocytes among all peripheral hemocytes (total DAPI count). (C) Confocal images of peripheral hemocytes from WT, abi5/Df, and HmlΔ-GAL4,abi5/UAS-HA-abi,Df (abi5/Df, HmlΔ > abi) late-third instar larvae stained with an anti-Lz antibody (green) and DAPI (blue). Arrowheads indicate Lz+ crystal cells. (D) Percentage of Lz+ crystal cells among all peripheral hemocytes (total DAPI count). abi5/Df; eater > abi and abi5/Df; lz > abi represent eater-GAL4,abi5/UAS-HA-abi,Df and lz-GAL4/+; UAS-HA-abi,Df/abi5, respectively. (E) Bright-field images of heated (70°C, 10 min) WT, abi5/Df, and abi5/Df, HmlΔ > abi late-third instar larvae. Dorsal views of the two most posterior segments (A7 and A8). (F) Numbers of heat-blackened crystal cells in the A7 and A8 segments of the indicated genotypes. (G) Confocal images of peripheral hemocytes from WT, abi5/Df, and abi5/Df, HmlΔ > abi late-third instar larvae stained with an anti-L1 antibody (green) and DAPI (blue). Arrowheads indicate L1+ lamellocytes. (H) Percentage of L1+ lamellocytes among all peripheral hemocytes (total DAPI count). abi5/Df, MSNF9 > abi represents MSNF9-GAL4/+; UAS-HA-abi,Df/abi5. (I) Confocal images of primary LG lobes from WT, abi5/Df, and abi5/Df, HmlΔ > abi late-third instar larvae stained with an anti-Lz antibody (green). Optical sections through the middle and two adjacent planes were projected. The dashed lines outline the MZ and CZ of the LG. (J) Numbers of Lz+ crystal cells in the primary LG lobes for the indicated genotypes. Data represent the mean ± SEM. n = 12 larvae/LG lobes. Statistical analyses were performed using Student’s t test (B) or a one-way ANOVA with the Tukey–Kramer post hoc test (D, F, H, and J). Comparisons are with WT (***P < 0.001; ns, not significant). Scale bars: 20 μm (A, C, G, and I); 200 μm (E). MZ, medullary zone; CZ, cortical zone.

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