TET2 ^Mut -related CHIP in aged individuals is associated with severe liver fibrosis and elevated Ccl2/Ccl8 levels. (A) The mutation frequency of genes known to be drivers of clonal hematopoiesis in aged individuals. The top four most frequently mutated genes in CHIP for the current study population were as follows: DNMT3A, TET2, TP53, and ASXL1. (B) The severity of liver fibrosis in TET2^WT and TET2^Mut population with liver fibrosis. TET2^WT, n = 11; TET2^Mut, n = 3. (C and D) The different levels of Ccl2 (C) and Ccl8 (D) in peripheral blood of patients with DNMT3A, TET2, TP53, and ASXL1 mutations. TP53^Mut, n = 3; ASLX1^Mut, n = 1; DNMT3A^Mut, n = 2; TET2^Mut, n = 3. (E and F) The levels of Ccl2 (E) and Ccl8 (F) in peripheral blood of TET2^WT and TET2^Mut population with liver fibrosis. TET2^WT, n = 11; TET2^Mut, n = 3. Data are the accumulative results from at least two independent experiments (A–F). All data are shown as mean ± SD and were analyzed by two-tailed, unpaired Student’s t test (B, E, and F) or two-way ANOVA with Sidak’s multiple comparison test (C and D). ***P < 0.001; **P < 0.01; *P < 0.05; P > 0.05 not significant (ns).