The upregulated Ccl2 and Ccl8 secreted by Tet2 ^−/− MDMs promote MDMs intrahepatic accumulation in a positive feedback manner. (A) GSEA for cytokine production involved in immune response and cytokine production involved in inflammatory response signaling pathways in Tet2^WT-CCl₄ versus Tet2^ΔMye-CCl₄ littermates (n = 4–5 for each group). (B) KEGG analysis of DEGs in Tet2^WT-CCl₄ versus Tet2^ΔMye-CCl₄ littermates. (C) Expression profiles of C–C motif ligands detected by RNA sequence in livers of Tet2^WT-CCl₄ and Tet2^ΔMye-CCl₄ littermates (n = 5 for each group). (D and E) The effect of Tet2^ΔMye on Ccl2 levels in serum (D) and livers (E) of mice with or without liver fibrosis. pg/mg: Ccl2 levels per milligram of liver tissue (n = 4 for each group). (F and G) The Ccl8 levels in serum (F) and livers (G) of four groups of mice. pg/mg: Ccl8 levels per milligram of liver tissue (n = 4 for each group). (H and I) Changes in Ccl2 (H) and Ccl8 (I) levels in serum of scramble, WT-MT, and KO-MT mice (n = 4 for each group). (K) Detection of Ccl2 and Ccl8 expression in Tet2^+/+ and Tet2^−/− MDMs by flow cytometry (n = 5 for each group) and RT-PCR (n = 8 for each group). Data are the accumulative results from at least two independent experiments (K) or are representative of at least two independent experiments with similar results (D–J). All data are shown as mean ± SD and were analyzed by two-way ANOVA with Sidak’s multiple comparison test (B–G, J, and K) or one-way ANOVA with Tukey’s multiple comparison test (H and I). ***P < 0.001; **P < 0.01; *P < 0.05; P > 0.05 not significant (ns).