Expansion advantage of transplanted Tet2 ^−/− CD45.2 ⁺ CD11b ⁺ cells and change of immune cells after Clod-Lip treatment in Tet2 ^WT -CCl ₄ and Tet2 ^ΔMye -CCl ₄ mice. (A) Strategy for flow cytometric analysis of the proportion of CD45.2⁺ cells in the blood and liver of scramble, WT-MT, and KO-MT mice. (B) Representative graphs of CD45.2⁺ cells in peripheral blood and liver of scramble, WT-MT, and KO-MT mice. (C) Flow cytometry strategy and statistical analysis of CD45.2⁺ F4/80⁺ cell frequency in livers of scramble, WT-MT, and KO-MT mice (n = 4). (D) Flow cytometry strategy for MDMs frequency in liver tissue of scramble, WT-MT, and KO-MT mice. (E and F) Strategy for flow cytometry analysis of MDMs (F), (CD86⁺) M1, and (CD206⁺) M2 macrophages (G) in livers of PBS-Lip– or Clod-Lip–treated Tet2^WT-CCl₄ and Tet2^ΔMye-CCl₄ mice. M1 macrophages were defined as Gr1⁺CD11b⁺F4/80⁺CD86⁺; M2 macrophages were defined as Gr1⁺CD11b⁺ F4/80⁺CD206⁺ populations. (G and H) IF (scale bar, 400 μm) of M1 MDMs (CD86) (G) and M2 MDMs (CD206) (H) in livers of Tet2^WT-CCl₄ and Tet2^ΔMye-CCl₄ mice. Blue: DAPI, red: anti-F4/80, green: anti-CD86/CD163, and yellow: anti-Ly6c. (I) Treatment schedule of PBS-Lip or Clod-Lip in Tet2^WT-CCl₄ and Tet2^ΔMye-CCl₄ mice. (J) H&E staining of liver tissues treated with PBS-Lip or Clod-Lip in Tet2^WT-CCl₄ and Tet2^ΔMye-CCl₄ mice. (K) Statistical analysis of DEGs in the comparison of Tet2^WT versus Tet2^ΔMye mice and Tet2^WT-CCl₄ versus Tet2^ΔMye-CCl₄ mice. A twofold decrease or increase in gene expression was considered statistically significant between different groups (Tet2^WT, n = 4; Tet2^ΔMye, n = 4; Tet2^WT-CCl₄, n = 5; Tet2^ΔMye-CCl₄, n = 5). Data are representative of at least two independent experiments with similar results (B, D, G, and F). All data are shown as mean ± SD and were analyzed by one-way ANOVA with Tukey’s multiple comparison test (D). ***P < 0.001; **P < 0.01; *P < 0.05; P > 0.05 not significant (ns).