Inflammatory cytokine and IFN responses in PBMCs following RSV infection. PBMCs from the patient (P, red) and three controls (C, grey) were mock infected (mock) or infected with RSV (RSV-A strain Long, MOI 1). (A–G) Cells were harvested 24 h after treatment, and RNA was isolated and analyzed for intracellular RSV RNA (A), IFNB1 (B), CXCL10 (C), IL6 (D), and TNF (E) gene expression by RT-PCR. Cell culture supernatants were harvested and analyzed for CXCL10 (F) and TNF (G) protein by ELISA. Bars indicate the mean ± SD values of a single experiment performed in triplicate. Statistics were calculated using the ordinary two-way ANOVA with Šídák’s multiple comparisons. ns = not significant. SD, standard deviation.