Figure 2.
Hyperinflammatory cytokine and IFN responses in patient PBMCs. (A–H) PBMCs from the patient (red) and three controls (grey) were left untreated (UT), stimulated with TLR4 agonist LPS, TLR7/8 agonist R848, transfected with PolyIC for TLR3/MDA5/RIG-I stimulation, or mock transfected (transf ctrl). Cells were harvested 24 h after treatment, and RNA was isolated and analyzed for IFNB1 (B), CXCL10 (C), IL6 (D), and TNF (E) gene transcription by RT-PCR, and cell culture supernatants were collected and analyzed for CXCL10 (F), IL6 (G), and TNF (H) protein by ELISA. Bars indicate mean values ± standard deviation (SD) of a single experiment performed in triplicates. Statistics were calculated using the ordinary One-way ANOVA with Šídák’s multiple comparisons test. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001, and ns = not significant.

Hyperinflammatory cytokine and IFN responses in patient PBMCs. (A–H) PBMCs from the patient (red) and three controls (grey) were left untreated (UT), stimulated with TLR4 agonist LPS, TLR7/8 agonist R848, transfected with PolyIC for TLR3/MDA5/RIG-I stimulation, or mock transfected (transf ctrl). Cells were harvested 24 h after treatment, and RNA was isolated and analyzed for IFNB1 (B), CXCL10 (C), IL6 (D), and TNF (E) gene transcription by RT-PCR, and cell culture supernatants were collected and analyzed for CXCL10 (F), IL6 (G), and TNF (H) protein by ELISA. Bars indicate mean values ± standard deviation (SD) of a single experiment performed in triplicates. Statistics were calculated using the ordinary One-way ANOVA with Šídák’s multiple comparisons test. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001, and ns = not significant.

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