Figure 7.
p62 and ATG7 are required for La secretion. (A) Immunoblot analysis of MDA-MB-231 cells expressing HA-CD63-mEGFPECL1, ZIM3-KRAB-dCas9, and either a nontargeting (sgNT) or p62/SQSTM1-targeting (sgSQSTM1) sgRNA. (B) Immunoblot analysis of exosomes immunoisolated from the conditioned medium of MDA-MB-231 cells expressing HA-CD63-mEGFPECL1, ZIM3-KRAB-dCas9, and either sgNT or sgSQSTM1. (C) Quantification of exosomal La secretion from sgNT and sgSQSTM1 cells. Statistical significance was performed using an unpaired two-tailed t test (n = 3; **P < 0.01). (D) Immunoblot analysis of HEK293T WT and ATG7 KO cells. (E) Immunoblot analysis of HSP fractions isolated from the conditioned medium of HEK293T WT and ATG7 KO cells. (F) Quantification of La, p62, LC3-II, and ALIX secretion from WT and ATG7 KO cells. Statistical significance was performed using unpaired two-tailed t tests (n = 3; **P < 0.01; ***P < 0.001; and ns, not significant). (G) Immunoblot analysis of HEK293T WT and FIP200 KO cells. (H) Immunoblot analysis of HSP fractions isolated from the conditioned medium of HEK293T WT and FIP200 KO cells. (I) Quantification of La, p62, LC3-II, and ALIX secretion from WT and FIP200 KO cells. Statistical significance was performed using unpaired two-tailed t tests (n = 3; ns, not significant). Source data are available for this figure: SourceData F7.

p62 and ATG7 are required for La secretion. (A) Immunoblot analysis of MDA-MB-231 cells expressing HA-CD63-mEGFPECL1, ZIM3-KRAB-dCas9, and either a nontargeting (sgNT) or p62/SQSTM1-targeting (sgSQSTM1) sgRNA. (B) Immunoblot analysis of exosomes immunoisolated from the conditioned medium of MDA-MB-231 cells expressing HA-CD63-mEGFPECL1, ZIM3-KRAB-dCas9, and either sgNT or sgSQSTM1. (C) Quantification of exosomal La secretion from sgNT and sgSQSTM1 cells. Statistical significance was performed using an unpaired two-tailed t test (n = 3; **P < 0.01). (D) Immunoblot analysis of HEK293T WT and ATG7 KO cells. (E) Immunoblot analysis of HSP fractions isolated from the conditioned medium of HEK293T WT and ATG7 KO cells. (F) Quantification of La, p62, LC3-II, and ALIX secretion from WT and ATG7 KO cells. Statistical significance was performed using unpaired two-tailed t tests (n = 3; **P < 0.01; ***P < 0.001; and ns, not significant). (G) Immunoblot analysis of HEK293T WT and FIP200 KO cells. (H) Immunoblot analysis of HSP fractions isolated from the conditioned medium of HEK293T WT and FIP200 KO cells. (I) Quantification of La, p62, LC3-II, and ALIX secretion from WT and FIP200 KO cells. Statistical significance was performed using unpaired two-tailed t tests (n = 3; ns, not significant). Source data are available for this figure: SourceData F7.

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