Figure S3.
Recombinant protein purification. (A) Coomassie-stained SDS-PAGE gel showing anti-FLAG–bound full-length and truncated La proteins prior to elution using 3xFLAG peptide. Asterisks (*) indicate anti-FLAG antibody fragments that were eluted from the resin upon denaturing elution with 1X Laemmli buffer (reducing). (B) Coomassie-stained SDS-PAGE gel showing the purification of recombinant p62. Source data are available for this figure: SourceData FS3.

Recombinant protein purification. (A) Coomassie-stained SDS-PAGE gel showing anti-FLAG–bound full-length and truncated La proteins prior to elution using 3xFLAG peptide. Asterisks (*) indicate anti-FLAG antibody fragments that were eluted from the resin upon denaturing elution with 1X Laemmli buffer (reducing). (B) Coomassie-stained SDS-PAGE gel showing the purification of recombinant p62. Source data are available for this figure: SourceData FS3.

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