Re-expression of FOXO1 at the peak of the GC reaction fully restores the FOXO1-associated transcriptional program in ZsGreen ⁺ rescued GCBCs. (A–E) ZsGreen⁺ and ZsGreen⁻ GCBCs from experimental and control mice were sorted at day (d)14 after immunization (TAM d10–12) to perform RNA sequencing. (A) Experimental scheme (left) and overview of mouse genotypes (right, n = 2–3 mice per genotype). (B) PCA from the 500 most varying genes. Each dot represents one sample. (C) Heatmap showing Pearson’s correlation coefficients between log₂ fold changes in gene expression profiles. (D and E) GSEA using published up- and downregulated Foxo1 (D) and DZ (E) gene signatures (Victora et al., 2012; Dominguez-Sola et al., 2015). Statistics: CERNO test. ES, enrichment score.