Characterization of the Cγ1-CDE strain. (A–E) Representative gating strategies for non-autofluorescence living single cells (A) and reporter-positive GCBCs (B)—including DZ and LZ GCBCs; IgG1⁺ MBCs (C); PCs (D); and IgG1⁺/IgM⁺ GCBCs (E). (F) Quantification of E at day 7—TAM day 2–5 (left panel); day 14—TAM day 9–12 (middle panel); and day 21—TAM day 15–18 (right panel) after NP-CGG immunization. (G) Percentage of Cre-mediated BFP⁺ (left panel) and Dre-mediated ZsGreen⁺ (within the BFP⁺) cells (right panel) among Fas⁺CD38⁻ GCBCs in PPs. (H) Percentage of Dre-mediated ZsGreen⁺ (within the BFP⁻) cells among splenic Fas⁺CD38⁻ GCBCs, splenic Fas⁻CD38⁺IgD⁻IgG1⁺ MBCs, and CD138⁺TACI⁺ PCs from spleen and BM (from one femur and tibia) at day 7—TAM day 2–5 (upper panel); day 14—TAM day 9–12 (middle panel); and day 21—TAM day 15–18 (lower panel). Statistics: Mann–Whitney test, **P ≤ 0.01; ***P ≤ 0.001; ns, not significant. Each dot represents one mouse. Data are from at least two independent experiments. Horizontal lines indicate the median.