Figure 5.
Figure 5. Pf% for P2X4-pHluorin123 receptors in relation to other P2X receptors. (A and B) The top panels show waveforms for ATP-evoked currents (black), charge (red), and changes in the fluorescence of FURA-2 (red) for WT P2X4 and P2X4-pHluorin123 receptors expressed in HEK-293 cells. The bottom graphs plot the change in fluorescence of FURA-2 against charge transfer. The slope of this graph provides the Pf(%) value. In these graphs, the change in fluorescence intensity of FURA-2 is shown in “bead units” (see Materials and methods). (C) Summary bar graph showing the average Pf(%) value for WT mP2X4 and mP2X4-pHluorin123 in relation to other P2X receptors. The values were compared using a Tukey–Kramer multiple comparisons ANOVA test. rP2X4, hP2X4, and zfP2X4 were significantly different to mP2X4 (*, P < 0.05), but P2X4-pHluorin123 was not (P > 0.05). Error bars represent mean ± SEM.

Pf% for P2X4-pHluorin123 receptors in relation to other P2X receptors. (A and B) The top panels show waveforms for ATP-evoked currents (black), charge (red), and changes in the fluorescence of FURA-2 (red) for WT P2X4 and P2X4-pHluorin123 receptors expressed in HEK-293 cells. The bottom graphs plot the change in fluorescence of FURA-2 against charge transfer. The slope of this graph provides the Pf(%) value. In these graphs, the change in fluorescence intensity of FURA-2 is shown in “bead units” (see Materials and methods). (C) Summary bar graph showing the average Pf(%) value for WT mP2X4 and mP2X4-pHluorin123 in relation to other P2X receptors. The values were compared using a Tukey–Kramer multiple comparisons ANOVA test. rP2X4, hP2X4, and zfP2X4 were significantly different to mP2X4 (*, P < 0.05), but P2X4-pHluorin123 was not (P > 0.05). Error bars represent mean ± SEM.

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