β3-AAA nAChRs show increased density in Golgi despite no change in ER export. Confocal image of α6-eGFPβ2β3 and Sec24D-mCherry (A), α6-eGFPβ2β3 with Golgi marker GalT-mCherry (C), and α6-eGFPβ2β3 nAChRs stained for endogenous β-COP (E). Quantification of: Sec24D fluorescence in ERES per cell for α6-eGFPβ2β3WT and α6-eGFPβ2β3AAA nAChRs (B) (n = 21–31); Pearson correlation between α6-eGFP* and GalT-mCherry fluorescence for α6-eGFPβ2β3WT and α6-eGFPβ2β3AAA nAChRs (D1) (n = 30–35); α6-eGFP* intensity in Golgi for α6-eGFPβ2β3WT and α6-eGFPβ2β3AAA nAChRs (D2) (n = 30–35); α6-eGFP fluorescence in COPI vesicles for α6-eGFPβ2β3WT and α6-eGFPβ2β3AAA nAChRs (F1) (n = 23–25); and Pearson correlation for the colocalization of α6-eGFP* fluorescence with COPI fluorescence in α6-eGFPβ2β3WT and α6-eGFPβ2β3AAA nAChRs (F2) (n = 23–24). Bars, 5 µm. *, P < 0.01; **, P < 0.005; ***, P < 0.0001 (ANOVA).
