The α6-SEP nAChRs are functional. Representative whole-cell ACh-induced currents of (A₁) α6-SEPβ2β3 nAChRs (3 µM ACh). (A₂) α6-eGFPβ2β3 nAChRs (3 µM ACh) and (A₃) α6-eGFPβ2 nAChRs (300 µM ACh). (B) Comparison of whole-cell ACh-induced current amplitudes of α6-SEPβ2β3, α6-eGFPβ2β3, and α6-eGFPβ2 nAChRs (n = 7–11). (C) Concentration–response of ACh on α6-eGFPβ2β3 nAChRs (n = 15). (D) Concentration-response of (−)-nicotine on α-eGFPβ2β3 nAChRs (n = 14). *, P < 0.05.