Analysis of Ca²⁺ signals in astrocyte territories. (A) The black bars represent the numbers of Ca²⁺ signals measured per Fluo-4AM bulk-loaded astrocyte as a function of distance from the soma (5-µm bins). The corresponding y axis is on the left. The gray line is for comparison and shows an average astrocyte Sholl plot (Reeves et al., 2011) (y axis on the right). (B and C) As in A, but for Ca²⁺ events detected from cells expressing Lck-GCaMP3 or cyto-GCaMP3. (D–F) Scatter graphs of Ca²⁺ event peak amplitudes (dF/F) as a function of distance from the somata.