Expression of cyto-GCaMP3 and Lck-GCaMP3 in astrocytes does not cause gliosis. (A–C) The top panels show the design of AAV2/5 constructs, and the bottom images show the results of IHC for Lck-GCaMP3, cyto-GCaMP3, and Lck-GFP with GFAP. (D) Summary bar graph showing areas of GFAP-labeled processes in relation to the areas of astrocytes expressing Lck-GCaMP3, cyto-GCaMP3, and Lck-GFP. (E and F) Summary graphs of GFAP-labeled areas and GFAP intensity for the experimental conditions indicated. *, P < 0.05 when compared with an unpaired Student’s t test.