Figure 5.

Steady-state force measured in myofibrils isolated from skeletal white muscles of the adult zebrafish determined at 10°C. (A) Force–pCa relationship of myofibrils pre-stretched 15% beyond their slack SLs; data are fitted to Eq. 2 (pCa50 and nH are given in Table I). (B) Passive force responses (at pCa 7.5) as a result of mechanical stretch at different successive SLs; data are fitted to the worm-like chain model of entropic elasticity. The inset shows the initial slope as an estimate of passive stiffness: 11.0 ± 1.3 kPa/µm (1 kPa = 1 nN/µm2). All values were normalized to the CSA of the myofibrils.

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