Summary of spatiotemporal Ca2+ spark parameters. Normalized amplitude, full duration at half magnitude (FDHM), and full-width at half magnitude (FWHM) are plotted versus time. Initially, all cells were in control solution, which was exchanged for test solution at the arrow. For composition of the test solution see symbol legend on the right. (A) [Mg2+]free was varied at constant ATPtotal (3 mM). (B) [ATP]free was varied at constant [Mg2+]free (0.2 mM). (C) [Mg2+]free was varied at constant [ATP]free (0.3 mM).