Ca2+ spark frequency is represented as a surface and is shown as a function of both [Mg2+]free and [ATP]free. The bivariate correlation coefficients obtained from a multivariate regression model for the Ca2+ spark frequency dependence on [Mg2+]free was −0.425 (P = 0.005) and on [ATP]free was +0.116 (P = 0.47). For details of the multivariate analysis see Materials and methods and Table S1.