Sample preparation for 10x single-cell RNA-Seq and cluster definition of B cells from WT and B-Fip200 ^−/− mice, related to Fig. 6. (A) Gating strategy of FACSorted plasma, GC, and memory B cell populations in IgD⁺ cell–depleted splenocytes from WT and B-Fip200^−/− mice. Naïve B cells (B220⁺IgD^hi) were sorted from intact splenocytes. (B) Statistical analysis of GC, plasma, and memory B cell populations in IgD⁺ cell–depleted splenocytes from WT and B-Fip200^−/− mice, as in Fig. 6 A. Significant P values were determined by an unpaired t test. Left to right: *P = 0.0316, *P = 0.0405, *P = 0.0268. (C) Heat map of top five expressed genes in 13 clusters in Fig. 6 A.