Figure S3.
FIP200 mediates mitophagy of TAX1BP1 binding mitochondria in B cells upon CD40L stimulation, related to Fig. 5. (A) Dynamic of the MitoQC-GFP expression level in WT and MitoQC-B-Fip200−/− B cells upon IL4+CD40L stimulation for 3 days. Data are representative of at least two independent experiments with three mice in each group. (B) Gating strategy of Live/single/focused/GFP+mCherry+ cells in ImageStream. (C) Representative plots of BDS score of MitoQC-B cells treated with FCCP or control (DMSO) for 30 min. Data are representative of at least two independent experiments; data from naïve B cells from one mouse per treatment are shown. (D) Representative plots of BDS score of WT and MitoQC-B-Fip200−/− B cells upon IL4+CD40L stimulation for 3 days; data were analyzed by the Kolmogorov–Smirnov test, ****P < 0.0001. The experiment was performed independently three times, with one representative experiment shown. n = 2–3. (E) Expression of TAX1BP1 was detected in WT and Fip200−/− B cells upon IL4+CD40L stimulation for 3 days. Two biological replicates were performed for WT and Fip200−/−; results from one shown. (F) Representative immunofluorescence Airyscan images with 90x magnification (Plan-Apochromat 50×/1.2 W objective, 1.8× magnification changer) of WT (n = 2; one shown) and MitoQC-B-Fip200−/− (n = 2; one shown) B cells activated by IL4+CD40L at day 2 and stained with anti-TAX1BP1 antibody (blue), MitoQC-GFP (green), and MitoQC-mCherry (red). Scale bar, 3 μm. Yellow arrows point to TAX1BP1 aggregation in B cells. Source data are available for this figure: SourceData FS3.

FIP200 mediates mitophagy of TAX1BP1 binding mitochondria in B cells upon CD40L stimulation, related to Fig. 5. (A) Dynamic of the MitoQC-GFP expression level in WT and MitoQC-B-Fip200−/− B cells upon IL4+CD40L stimulation for 3 days. Data are representative of at least two independent experiments with three mice in each group. (B) Gating strategy of Live/single/focused/GFP+mCherry+ cells in ImageStream. (C) Representative plots of BDS score of MitoQC-B cells treated with FCCP or control (DMSO) for 30 min. Data are representative of at least two independent experiments; data from naïve B cells from one mouse per treatment are shown. (D) Representative plots of BDS score of WT and MitoQC-B-Fip200−/− B cells upon IL4+CD40L stimulation for 3 days; data were analyzed by the Kolmogorov–Smirnov test, ****P < 0.0001. The experiment was performed independently three times, with one representative experiment shown. n = 2–3. (E) Expression of TAX1BP1 was detected in WT and Fip200−/− B cells upon IL4+CD40L stimulation for 3 days. Two biological replicates were performed for WT and Fip200−/−; results from one shown. (F) Representative immunofluorescence Airyscan images with 90x magnification (Plan-Apochromat 50×/1.2 W objective, 1.8× magnification changer) of WT (n = 2; one shown) and MitoQC-B-Fip200−/− (n = 2; one shown) B cells activated by IL4+CD40L at day 2 and stained with anti-TAX1BP1 antibody (blue), MitoQC-GFP (green), and MitoQC-mCherry (red). Scale bar, 3 μm. Yellow arrows point to TAX1BP1 aggregation in B cells. Source data are available for this figure: SourceData FS3.

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