CXCR4 activation reprograms the behavior of intravascular neutrophils. (a) UMAP plots showing the four distinct behavioral clusters identified by 4D IVM (left) labeled with colors and distribution of neutrophils from WT ZT9 and ZT17, platelet-depleted, and ATI-treated mice (right). (b) Behavioral profiling: heatmap of the 73 morphokinetic parameters obtained for neutrophils inside inflamed venules and distributed for the four identified behavioral profiles. (c) Volumetric reconstruction of representative cells from behavioral clusters (top) and temporal outlines (bottom). (d) Violin plots for the indicated parameters across the four behavioral groups. (e) UMAP (left) and doughnut plots (right) showing the distributions of neutrophils in each experimental group for each behavioral cluster. Numbers are the frequency of intravascular neutrophils with a B3 pathogenic profile. Data in a–e are pooled from cells analyzed from two experiments. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant, as determined by one-way ANOVA in d. IVM, intravital microscopy.