Circadian activity and blood counts of neutrophil-depleted, CXCR4, and Bmal1 mutant mice. (a) Myeloid cells and neutrophils at the indicated circadian times in the blood of WT, Bmal1^ΔN, Cxcr4^+/1013 mice; n = 3–4 mice per group. (b) Peripheral blood counts in WT littermates, Cxcr4^+/1013 (WHIM) (up), and Bmal1^ΔN (bottom) mice at ZT5; n = 7–9 mice per group for Cxcr4^+/1013 and n = 5 mice per group for Bmal1^ΔN. (c) O₂ consumption (VO2), CO₂ production (VCO2), energy expenditure (EE), respiratory quotient (RQ), general locomotor activity (activity), vertical activity (rearing), food intake (food), and drink intake (drink) of WT, Bmal1^ΔN, Cxcr4^+/1013, and neutrophil-depleted mice housed in metabolic cages for 3 days with food and water available ad libitum; n = 3–14 mice per group. AUs: arbitrary units. (d) Experimental scheme for evaluation of cardiac function by MRI in WT and Bmal1^ΔN after performing AMI (45′ occlusion; 24-h reperfusion) at the indicated ZTs. (e) EF measured by MRI at day 0 and day 7 in WT and Bmal1^ΔN mice subjected to AMI and followed for 24 h of reperfusion at the indicated ZTs; n = 3–4 mice per group. (f) Difference in EF (ΔEF) between day 0 and day 7 in mice from e; n = 3–4 mice per group. Data in a and c–f are from single experiments. Data in b (bottom; Cxcr4^+/1013) are pooled from two experiments. Data in b (up; Bmal1^∆N) are representative of two experiments. Data are shown as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant, as determined by unpaired t test analysis (b and f) and two-way ANOVA (a, c, and e).