Comparison of GALT FRC with other lymphoid tissue FB and with their counterparts during CD. (A) UMAPs showing the intestinal stromal cell populations described by Kong et al. (2023), Kinchen et al. (2018), and Elmentaite et al. (2021) based on the original annotations. (B) Heatmap showing CD24 expression by the FB clusters described in Kinchen et al. (2018) and Elmentaite et al. (2021). (C) UMAPs showing the expression of the indicated GALT FRC subset signatures (top 10 DEG per cluster) or CD24 expression on the tonsillar FRC dataset published by De Martin et al., as well as the original tonsillar FRC clustering (De Martin et al., 2023). ACTA2⁺ PRC = α-smooth muscle actin–expressing PRC; PI16⁺ RC = peptidase inhibitor 16–expressing reticular cells. (D) Confocal laser microscopy images of mesenteric LN sections showing the expression of CD24 on PDPN⁺ FB and CD45⁺ immune cells. The boxes indicate magnified areas shown in the middle (yellow) and right panel (turquoise). Star marks a lymphatic sinus, yellow arrowheads indicate PDPN⁺CD24⁺ FRC. One image shown of three replicates from different donors that were stained in independent experiments. (E) Proportions of GALT FRC subsets in the indicated samples. The numbers above each bar indicate the number of GALT FRC per dataset.