FRC landscape of large intestinal SM-ILF and transcriptional differences to ileal GALT. (A) Bar plots showing the abundance of the GALT FRC subclusters among all GALT FRC from large intestinal SM-ILF of five independently processed donors. (B) Confocal laser microscopy images showing the abundance of CD3⁺ T cells, CD19⁺ B cells (left panel), and CD24-expressing FRC among all PDPN⁺ FB (right panel) within large intestinal SM-ILF. (C) Confocal laser microscopy images showing the expression of CD24 on F3⁺/CR1/2⁺/COLVI⁺ FRC within large intestinal SM-ILF. (D) Confocal laser microscopy images showing the expression of CD24 on F3⁺/CR1/2⁺/COLVI⁺ FRC within large intestinal SM-ILF. Star marks the apical side of the epithelial layer facing the intestinal lumen. (E) Confocal laser microscopy images showing the expression of CXCL13 levels by FRC in the SED region. Yellow arrowheads indicate location of F3⁺ SED FRC. (F) Confocal laser microscopy images showing the expression of HLA-DR in large intestinal SM-ILF. Yellow arrowheads indicate location of PDPN⁺HLA-DR⁺ FRC. (B–F) Images show one representative image out of two or three replicates that were stained in independent experiments. (G) Violin plots showing the expression of the indicated genes by large intestinal SM-ILF, as well as ileal PP and M-ILF. Data are from five independently processed tissue donors (5× PP and SM-ILF, 3× M-ILF).