Figure 1. scRNA-seq reveals four... | Rockefeller University Press

Figure 1.

$scRNA-seq reveals four distinct intestinal FB clusters. (A) Schematic overview showing the anatomic sites of the human intestine from which samples were obtained. (B) Left panel: Representative stromal cell gate showing CD31+PDPN− vascular endothelial cells (red), CD31+PDPN+ lymphatic endothelial cells (blue), CD31−PDPN+ FB (green), and the CD31−PDPN− double negative cells (gray). Right panel: Quantification of the stromal cell fractions in the indicated isolated intestinal layers. The plot shows the means ± SD of 20 (large intestinal LP and SM-ILF), 17 (ileal LP and PP), 10 (large intestinal SM), 6 (ileal SM), or 4 (ileal M-ILF) tissue donors; pooled data are shown from 19 independent experiments. (C) UMAP representation of unsupervised Louvain clustering of 101,401 intestinal FB pooled from the LP, SM, and GALT. (D) Heatmap showing the top ten DEG for the four identified FB clusters. (E) Heatmap showing expression levels of canonical FB markers by the four identified intestinal clusters. (F) Pie chart representation of FB cluster abundance in the scRNA-seq data object at the indicated sites. Results in C–F are pooled from five (PP and SM-ILF), three (ileal and large intestinal LP and M-ILF), and one (ileal and large intestinal SM) tissue donors.$

scRNA-seq reveals four distinct intestinal FB clusters. (A) Schematic overview showing the anatomic sites of the human intestine from which samples were obtained. (B) Left panel: Representative stromal cell gate showing CD31⁺PDPN⁻ vascular endothelial cells (red), CD31⁺PDPN⁺ lymphatic endothelial cells (blue), CD31⁻PDPN⁺ FB (green), and the CD31⁻PDPN⁻ double negative cells (gray). Right panel: Quantification of the stromal cell fractions in the indicated isolated intestinal layers. The plot shows the means ± SD of 20 (large intestinal LP and SM-ILF), 17 (ileal LP and PP), 10 (large intestinal SM), 6 (ileal SM), or 4 (ileal M-ILF) tissue donors; pooled data are shown from 19 independent experiments. (C) UMAP representation of unsupervised Louvain clustering of 101,401 intestinal FB pooled from the LP, SM, and GALT. (D) Heatmap showing the top ten DEG for the four identified FB clusters. (E) Heatmap showing expression levels of canonical FB markers by the four identified intestinal clusters. (F) Pie chart representation of FB cluster abundance in the scRNA-seq data object at the indicated sites. Results in C–F are pooled from five (PP and SM-ILF), three (ileal and large intestinal LP and M-ILF), and one (ileal and large intestinal SM) tissue donors.

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