Patronin and acetylated MT intensities in EB1-disrupted embryos. (A) Orthogonal view of acetylated MTs color coded for intensity levels in control and ΕΒ1 embryos, and quantification is shown in (A′) (mean ± SD); n = 70 and 60 regions for control and EB1, respectively, from k = 7 and 6 embryos for control and EB1, respectively. (B) Orthogonal projection showing Patronin intensities at perinuclear regions (arrows) in control and EB1 embryos. (C) Peak nuclear speeds in control and EB1 embryos; n = 546 and 383 nuclei for control and EB1, respectively. Scale bar = 5 μm. Statistical significance was calculated using the Mann–Whitney U-test. ns, not significant. ****P < 0.0001.