Nuclear behaviors after γ-tub disruption. (A) Nuclei color-coded for apical–basal position in control and γ-tub embryos. (B) Fraction of nuclei in apical 10 μm of cell in control and γ-tubulin embryos; n = 263 and 273 nuclei for control and γ-tub, respectively, k = 3 embryos each background. (C) Mean nuclear speeds in control and γ-tub embryos. (D) Peak nuclear speeds in control and γ-tub embryos; n = 546 and 612 in control and γ-tub, respectively, from k = 3 embryos for each background. (E) Percent of active nuclei as detected by MSD in control and γ-tub embryos. (C and E) n = 546 and 1,223 nuclei in control and γ-tub, respectively, from k = 3 embryos for each background. Scale bar = 10 μm. Statistical significance was calculated using the Mann–Whitney U-test. *P < 0.05, ****P < 0.0001.